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KMID : 1101220000320030279
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2000 Volume.32 No. 3 p.279 ~ p.286
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Maintenance of Transmission Electron Microscope(TEM) by the Observation of Digestive System of Urechius unicinctus
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Lim Hyung-Seon
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Abstract
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Like that of the vertebrates, cilia in the digestive system of urechis unicinctus consist of one pair of microtubles in the middle and the circumferential nine pairs of microtubles that form a circle around it. Considering much glycogen in the cytoplasm, the cells did not have cilia. Muscles of the digestive system is located at the lower area of the epidermal layer of the middle gut. The secreted granules and the distribution of nuclei were in inverse proportion, and the concentration of the secreted granules can be determined with the formation of a streak-shaped film. on the free surfaces of the epithelial cells in the digestive system are the basal bodies, microtubles and macula adherens, which enable the bonding with the surrounding cells. ThiS microstructure of the cells can be observed when an electron microscope is in perfect installation conditions. The conditions include exclusive power source and the deviation of voltage within 10%. A stable magnetic field (0.3 μ T or less for AC and 10mG or less for DC) is required and the water supply should have the pressure at 0.1-0.3MPa (1-3kg?min=0.5-2kg?cm2), the flow of 4 liter?min, and the temperature of 15-20ºC, to allow for easy movement of electrons and to cool the lenses. The maximum tolerable vibration is 40dB or so and the temperature (25ºC) and humidity (70% or lower) should be constantly maintained. The inside of the electron microscope should be maintained at a pre-vacuum condition (10-3-10-5 Torr) using a rotary pump.
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KEYWORD
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Install environment, Transmission electron microscope(TEM), Urechius unicinctus
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Listed journal information
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